Spot 42 RNA

Spot 42 RNA
Predicted secondary structure and sequence conservation of Spot_42
Identifiers
Symbol Spot_42
Alt. Symbols Spot42
Rfam RF00021
Other data
RNA type Gene; sRNA
Domain(s) Bacteria
SO 0000389

The Spot 42 (spf) RNA is a non-coding RNA molecule discovered over three decades ago.[1] Escherichia coli Spot 42 RNA is 109 nucleotides in length. Its physiological role has remained obscure, but Spot 42 seems to have a regulatory role on the galactose operon.[2] It is proposed that Spot 42 acts by an antisense mechanism where the RNA binds to the galK translation initiator region. Changes in Spot 42 levels are implicated in affecting the adjacent DNA polymerase I levels. [3] The Hfq protein has been shown to interact with several small regulatory RNAs in E. coli, and the protein is required for OxyS, DsrA, RprA and Spot 42 RNA regulation. [4] Transcription of spot 42 is repressed by the CRP protein.[5]

Further targets, that have been discovered, imply an extensive role for Spot 42 RNA in catabolite repression. Evidence suggests that Spot 42 RNA posttranscriptional positive regulator of gsp and dppB and a negative regulator of several genes including fucK, galK, fucl and ebgC.[6][7]

References

  1. ^ Sahagan BG, Dahlberg JE (July 1979). "A small, unstable RNA molecule of Escherichia coli: spot 42 RNA. I. Nucleotide sequence analysis". J. Mol. Biol. 131 (3): 573–92. doi:10.1016/0022-2836(79)90008-1. PMID 390161. 
  2. ^ Moller, T; Franch T, Udesen C, Gerdes K, Valentin-Hansen P (2002). "Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon". Genes Dev 16 (13): 1696–1706. doi:10.1101/gad.231702. PMC 186370. PMID 12101127. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=186370. 
  3. ^ Polayes, DA; Rice PW, Dahlberg JE (1988). "DNA polymerase I activity in Escherichia coli is influenced by spot 42 RNA". J Bacteriol 170 (5): 2083–2088. PMC 211090. PMID 2452153. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=211090. 
  4. ^ Moller, T; Franch T, Hojrup P, Keene DR, Bachinger HP, Brennan RG, Valentin-Hansen P (2002). "Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction". Mol Cell 9 (1): 23–30. doi:10.1016/S1097-2765(01)00436-1. PMID 11804583. 
  5. ^ Polayes DA, Rice PW, Garner MM, Dahlberg JE (July 1988). "Cyclic AMP-cyclic AMP receptor protein as a repressor of transcription of the spf gene of Escherichia coli". J. Bacteriol. 170 (7): 3110–4. PMC 211256. PMID 2454912. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=211256. 
  6. ^ Beisel CL, Storz G (2011). "The Base-Pairing RNA Spot 42 Participates in a Multioutput Feedforward Loop to Help Enact Catabolite Repression in Escherichia coli.". Mol Cell 41 (3): 286–97. doi:10.1016/j.molcel.2010.12.027. PMID 21292161. 
  7. ^ Papenfort K, Vogel J (2011). "Sweet Business: Spot42 RNA Networks with CRP to Modulate Catabolite Repression.". Mol Cell 41 (3): 245–6. doi:10.1016/j.molcel.2011.01.011. PMID 21292156. 

External links